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Author
- Ntiamoah, Peter (1)
- Ohnishi, Chie (1)
- Ohnishi, Takashi (1)
Subject
- CCS (1)
Date issued
- 2023 (1)
Has File(s)
- true (1)
Search Results
In the evaluation of human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) — one of the standard biomarkers for breast cancer— visual assessment is laborious and subjective. Image analysis using whole slide image (WSI) could produce more consistent results; however, color variability in WSIs due to the choice of stain and scanning processes may impact image analysis. We therefore developed a calibration protocol to diminish the staining and scanning variations of WSI using two calibrator slides. The IHC calibrator slide (IHC-CS) contains peptide-coated microbeads with different concentrations. The color distribution obtained from the WSI of stained IHC-CS reflects the staining process and scanner characteristics. A color chart slide (CCS) is also useful for cali... |