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Title: Standardizing HER2 immunohistochemistry assessment: calibration of color and intensity variation in whole slide imaging caused by staining and scanning
Authors: Ohnishi, Chie
Ohnishi, Takashi
Ntiamoah, Peter
Issue Date: 2023
Publisher: Springer
Abstract: In the evaluation of human epidermal growth factor receptor 2 (HER2) immunohistochemistry (IHC) — one of the standard biomarkers for breast cancer— visual assessment is laborious and subjective. Image analysis using whole slide image (WSI) could produce more consistent results; however, color variability in WSIs due to the choice of stain and scanning processes may impact image analysis. We therefore developed a calibration protocol to diminish the staining and scanning variations of WSI using two calibrator slides. The IHC calibrator slide (IHC-CS) contains peptide-coated microbeads with different concentrations. The color distribution obtained from the WSI of stained IHC-CS reflects the staining process and scanner characteristics. A color chart slide (CCS) is also useful for calibrating the color variation due to the scanner.
Description: CC-BY
URI: https://link.springer.com/article/10.1186/s42649-023-00091-8
https://dlib.phenikaa-uni.edu.vn/handle/PNK/9064
Appears in CollectionsOER - Khoa học Vật liệu, Ứng dụng
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